Stable Delivery of a Canavalin Promoter- -Glucuronidase Gene Fusion into French Bean by Particle Bombardment
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چکیده
منابع مشابه
Stable Transformation of Saintpaulia ionantha by Particle Bombardment
Background: A highly efficient genetic transformation system is essential for a successful genetic manipulation of the African violet (Saintpaulia ionantha Wendl.). Objectives: Developing a particle bombardment-based genetic transformation system for the African violet. Materials and Methods: A local cultivar of the African violet from Guilan province was used for transformation experiments. Th...
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The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion. We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes. The 5'-flanking sequence and part of the structural region of an oat p...
متن کاملstable transformation of saintpaulia ionantha by particle bombardment
background: a highly efficient genetic transformation system is essential for a successful genetic manipulation of the african violet (saintpaulia ionantha wendl.).objectives: developing a particle bombardment-based genetic transformation system for the african violet.materials and methods: a local cultivar of the african violet from guilan province was used for transformation experiments. the ...
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Transfection of trypanosomatids, particularly the transfection of their mitochondrion and different developmental stages, is still a limiting step in many experiments using recombinant DNA technology. Two types of trypanosomatid transfection protocols have been described so far. The standard protocol is based on electroporation first described by Bellofatto and Cross [1]. The second protocol is...
متن کاملStable transformation of plant cells by particle bombardment/biolistics.
Particle bombardment, or biolistics, is a commonly used method for genetic transformation of plants and other organisms. Millions of DNA-coated metal particles are shot at target cells or tissues using a biolistic device or gene gun. The DNA elutes off the particles that lodge inside the cells, and a portion may be stably incorporated in the host chromosomes. A protocol for the generation of tr...
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ژورنال
عنوان ژورنال: Plant and Cell Physiology
سال: 1997
ISSN: 0032-0781,1471-9053
DOI: 10.1093/oxfordjournals.pcp.a029087